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ONE-STEP®
IN
VITRO TESTING PROTOCOL |
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In a clinical situation, keeping the dentin "moist"
is easy because of high humidity and three dimensional
cavity configuration. To simulate these conditions in
a laboratory situation (flat specimen), where even a
brief burst of air can remove too much moisture, the
researcher must pay particular attention to maintaining
a visibly "moist" dentin surface prior to adhesive applications.
PROTOCOL FOR PREPARING IN VITRO "MOIST" DENTIN BONDING
SPECIMEN:
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| 1. |
Apply UNI-ETCH®
with BAC (32% H3PO4) to tooth surface
for 15 seconds. Rinse thoroughly and store in distilled
water. |
| 2. |
Dispense 2 drops of ONE-STEP
Adhesive into a mixing well. Taking only one specimen
out of the water at a time, blot the tooth surface of
excess water with a "moist" cotton pellet or Kimwipes®
(laboratory wipes). |
| 3. |
With a fully saturated brush
tip, apply 2 consecutive coats to dentin (and/or enamel)
with slight agitation. |
| 4. |
Gently apply air for 10 seconds
to evaporate solvent. A smooth glossy surface should be
present. (If not shiny, apply an additional coat and evaporate
solvent). |
| 5. |
Light-cure adhesive for 10
seconds, bond composite to specimen. |
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